Reporter

Part:BBa_K1204016:Design

Designed by: Matthew Baron   Group: iGEM13_Duke   (2013-09-16)


TEF1pr-BS1x-20A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 176


Design Notes

Had to follow rules of iTAL and gRNA/dCas9 complex binding


Source

Ordered as gene block from IDT

References